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Capparis decidua and Selenium have several antibacterial properties along with other highly desirable properties, but for long they have not been explored. Nanoparticles are efficient in transporting drugs as they are easily absorbed by the body due to their small size. We wanted to evaluate the antibacterial activity of Capparis decidua mediated selenium nanoparticles against Streptococcus mutans, Lactobacillus, and Enterococcus faecalis. METHODS4 wells were dug in three Petri dishes of Agar which were coated with a layer of bacteria, Streptococcus mutans, Lactobacillus and Enterococcus faecalis. The wells were filled with different amounts of the extract in the order of 50 µL, 100 µL and 150 µL with the fourth well having 50 µL of the antibiotic. Zone of inhibition was measured. RESULTSAfter the measurements were made, the results obtained showed good zone of inhibition against Streptococcus mutans, Lactobacillus and Enterococcus faecalis. CONCLUSIONSThis study suggests that Capparis decidua fruit mediated with Selenium Nanoparticles have a high antibacterial activity.
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Origanum vulgare L. (OVEO) essential oil has been considered a candidate antimicrobial for use in food conservation systems. However, studies on the influence of concomitant variations of different food components or physicochemical parameters on the antibacterial properties of OVEO are scarce. This study assessed the influence of concomitant variations in amounts of proteins - PTN (4.0, 6.0 or 8.0 g/100 mL) and lipids - LIP (3.75, 5.0 or 6.25 g/100 mL) and pH values (5.0, 5.5 or 6.0) in cultivation medium on the inhibitory effects of OVEO against Escherichia coli (EC) and Salmonella Typhimurium (ST). Lowest minimum inhibitory concentration values of OVEO against EC and ST were observed in media with the highest LIP amounts regardless the PTN amount and pH value. In absorbance based microtiter plate assay (MPA), for both EC and ST, OVEO caused the lowest Grmax values in medium containing the highest LIP and PTN amounts and lowest pH value. Highest Grmax values for EC and ST were observed in medium containing the lowest LIP and PTN amount and highest pH value. Grmax values estimated from viable counts of EC and ST in tested media with OVEO confirmed bacterial growth behavior similar to that observed in MPA. Overall, the LIP amount in media was as the most influential factor to enhance the antibacterial effects of OVEO. These results indicate that the concomitant influence of LIP and PTN amounts and pH values on the antibacterial effects of OVEO should be considered for optimizing its antimicrobial efficacy in foods.
Subject(s)
Salmonella typhimurium/classification , Oils, Volatile/analysis , Origanum/classification , Escherichia coli/classification , Lipids/adverse effects , Proteins , Microbial Sensitivity Tests/instrumentation , Bacterial Growth , Efficacy , Food , Hydrogen-Ion ConcentrationABSTRACT
OBJECTIVES: Galla chinensis inhibited the adherence of planktonic oral bacteria and acid production by cariogenic bacteria. However, little is known about the relevant conditions of Galla Chinensis extract (GCE) exposure time and concentration and the effect of GCE on the structural and functional activity of cariogenic bacteria. The antibacterial effects of natural G. Chinensis extract on S. mutans , S. sanguinis, and S. oralis biofilms were evaluated in vitro.METHODS: Biofilms formed on glass surfaces were treated with different concentrations of GCE at different exposure times. The effects were assessed by examining the bactericidal activity, acidogenesis, minimum inhibitory concentration, and morphology.RESULTS: There was a statistically significant difference in the bacterial growth inhibition depending on the concentration of the GCE, with bacterial growth being inhibited as the concentration of GCE increased. A concentration of 1.0 mg/ml GCE had similar bactericidal effects against S. mutans and S. oralis biofilms to those produced by 2.0 mg/ml CHX. In the 1.0 mg/ml GCE group, incomplete septa were also observed in the outline of the cell wall, together with disruption of the cell membrane. In addition, there was also a slight exudation of the intracellular content from the bacteria in the 1.0 mg/ml GCE and 2 mg/ml CHX groups.CONCLUSIONS: These results indicate that GCE inhibits the growth of S. mutans, S. sanguinis, and S. oralis with increasing concentrations. It alters the microstructure of S. mutans biofilms. These results suggest that GCE might be a useful anti-bacterial agent for preventing dental caries.
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Bacteria , Biofilms , Cell Membrane , Cell Wall , Dental Caries , Glass , In Vitro Techniques , Microbial Sensitivity Tests , Plankton , Streptococcus mutansABSTRACT
Bacterial infection of the uterus in Hanwoo can kill embryos. Therefore, many antibiotics have been used to treat this infection in the uterus of repeat breeders. Incorrect use of antibiotics has led to resistance in bacteria. Natural compounds have used as substitutes for antibiotics because they are safe and have very mild side effects. This study was conducted to examine the antimicrobial effects of five extracts from medicinal plants including Humulus japonicas (Hj), Phelledendron amurense (Pa), Viola mandshurica (Vm), Carthamus tinctorius (Ct), and Chelidoni herba (Ch) on bacteria isolated from the uterus of Hanwoo using the paper disc diffusion method. Hj and Pa extracts had potent antimicrobial effects against Staphylococcus lentus, Streptococcus infantarius subsp. coli, and Bacillus pumilus. Pa had the greatest antimicrobial effect among the five medicinal plants and was effective against 19 types of bacteria from bovine uterus. Compared to Pa, Hj showed weaker antimicrobial effects on all the bacteria tested except Pseudomonas aeruginosa. Vm, Ct, and Ch also showed weak antimicrobial effects on the tested bacteria. The results obtained suggest that Hj and Pa are natural compounds suitable for treating bacterial infection in repeat breeders and improving conception rates of Hanwoo.
Subject(s)
Anti-Bacterial Agents , Bacillus , Bacteria , Bacterial Infections , Carthamus tinctorius , Diffusion , Embryonic Structures , Fertilization , Humulus , Plants, Medicinal , Pseudomonas aeruginosa , Staphylococcus , Streptococcus , Uterus , ViolaABSTRACT
Bacterial infection of the uterus in Hanwoo can kill embryos. Therefore, many antibiotics have been used to treat this infection in the uterus of repeat breeders. Incorrect use of antibiotics has led to resistance in bacteria. Natural compounds have used as substitutes for antibiotics because they are safe and have very mild side effects. This study was conducted to examine the antimicrobial effects of five extracts from medicinal plants including Humulus japonicas (Hj), Phelledendron amurense (Pa), Viola mandshurica (Vm), Carthamus tinctorius (Ct), and Chelidoni herba (Ch) on bacteria isolated from the uterus of Hanwoo using the paper disc diffusion method. Hj and Pa extracts had potent antimicrobial effects against Staphylococcus lentus, Streptococcus infantarius subsp. coli, and Bacillus pumilus. Pa had the greatest antimicrobial effect among the five medicinal plants and was effective against 19 types of bacteria from bovine uterus. Compared to Pa, Hj showed weaker antimicrobial effects on all the bacteria tested except Pseudomonas aeruginosa. Vm, Ct, and Ch also showed weak antimicrobial effects on the tested bacteria. The results obtained suggest that Hj and Pa are natural compounds suitable for treating bacterial infection in repeat breeders and improving conception rates of Hanwoo.
Subject(s)
Anti-Bacterial Agents , Bacillus , Bacteria , Bacterial Infections , Carthamus tinctorius , Diffusion , Embryonic Structures , Fertilization , Humulus , Plants, Medicinal , Pseudomonas aeruginosa , Staphylococcus , Streptococcus , Uterus , ViolaABSTRACT
ObjectiveTo investigate the anti-inflammatory effect and mechanism of Yijie particles. MethodsTook the energometry of SOD in foot of rats metatarsal inflammatory organization induced by egg white,Maked antibacterial experiment investigation in vitro to observe the anti-inflammatory effect of Yijie particles and observed mice abdominal macrophage phagocytosis function of red blood cells. ResultsCompared to the control group,the SOD of content in the large,medium and small dose group were statistically significant difference(all P＜0.01),the mice abdominal macrophage phagocytosis percentage of red blood cells and devour exponential increased significantly(all P＜0.01).4 kinds of pathogenic bacteria of the lowest antibacterial and minimum bactericidal concentration(MBC),respectively,streptococcus pneumoniae(MIC 0.286g/ml,MBC 0.429g/ml);Clay white fungus(MIC 0.140g/ml,MBC 0.275g/ml);Pseudomonas aeruginosa(MIC 0.0177g/ml),MBC 0.0358g/ml);E.coli(MIC 0.429g/ml,MBC 0.429g/ml). ConclusionYijie particles had remarkable anti-inflammatory and better antibacterial effect.
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PURPOSE: To evaluate an effect of controlled antibiotic release of the layered double hydroxides (LDH) as a new drug delivery system, and to observe a histological changes of the LDH in vivo and the differences of antibacterial effects among the several antibiotic-LDH hybrids. MATERIALS AND METHODS: Staphylococcus aureus (ATCC 23235) and Escherchia coli (ATCC 10536) were used as a bacterial specimens. Antibiotic-LDH hybrids were gentamicin-LDH (GM-LDH), cefaclor-LDH (CCLO-LDH), cefuroxime axetil-LDH (CRXMA-LDH) and ceftazidime-LDH (CAZ-LDH). In vitro study, two methods were used. One was dilution method, used to determine the minimum inhibitory concentrations (MICs) of the antibiotic-LDH hybrids. The other one was disk diffusion method, to observe the zones of bacterial inhibition of them. In vivo forty New Zealand White rabbits (2.5~3.0 kg) were divided into 10 groups. Animals were anesthetized and a 3 mm-diameter hole was drilled 2 cm proximal to the distal end of the left femur. The antibiotic-LDH hybrids was put into the drilled hole and then 0.1 ml (105 CFU/ml) of S. aureus and E. coli were inoculated into the drilled hole in each group. Histological examination was done at postoperative 1, 2, 3, and 4 weeks respectively. RESULTS: The MICs for S. aureus were more than 400 microgram/ml in GM-LDH, 25 microgram/ml in CRXMA-LDH, 25 microgram/ml in CCLO-LDH, and 100 microgram/ml in CAZ-LDH. The MICs for E. coli were 12.5 microgram/ml in GM-LDH, 25 microgram/ml in CRXMA-LDH, 25 microgram/ml in CCLO-LDH, and 1.56 microgram/ml in CAZ-LDH. CCLO-LDH was effective on both S. aureus and E. coli and CRXMA-LDH on E. coli in disk diffusion method. In contrast, GM-LDH and CAZ-LDH were not effective on neither S. aureus nor E. coli. Histologically, LDH was shown as large masses at the postoperative 1~2 weeks and changed to several small masses or fragments at the postoperative 3~4 weeks. CONCLUSION: There was much difference of the extent of controlled antibiotic release among antibiotic-LDH hybrids. The size and volume of LDH in vivo was reduced gradually. CCLO-LDH, and CRXMA-LDH seemed to have an effect of the bacterial growth inhibition.
Subject(s)
Animals , Rabbits , Cefuroxime , Diffusion , Drug Delivery Systems , Femur , Hydroxides , Microbial Sensitivity Tests , Staphylococcus aureusABSTRACT
OBJECTIVE To discuss the antibacterial and anti-inflammatory effects of GanKeQing granules.METHODS The antibacterial tests in vitro,and the tests of it′s affects for auricular swelling induced by xylene and celiac capillary permeability induced by acetic acid in mice were done to observe the pharmaceutical effects of GanKeQing granules.RESULTS GanKeQing granules could inhibit and kill 5 kinds of pathogenic bacteria,suppress the swelling of the mouse′s ear caused by xylene and releave the enhancment of mice capillary permeability caused by acetic acid.CONCLUSIONS GanKeQing granules have better antibacterial and anti-inflammatory effects,which could be used for the treatment and prevention of cold and flu.
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